CST, 72240S, CD69 (F8N6A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD69 mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CD69 (F8N6A) Rabbit Monoclonal Antibody (CST #72240) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD69 (F8N6A) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD69 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse CD69 protein. Background CD69, also known as Leu-23, is a type II transmembrane glycoprotein that is expressed on the surface of T cells, B cells, and NK cells (1,2). This phosphorylated disulfide-linked 28 to 32-kDa homodimer is constitutively expressed on a subset of thymocytes and platelets. It also acts as an activation antigen of lymphocytes, NK cells, neutrophils, and eosinophils (1-6). Studies have shown that stimulation of the T cell receptor (TCR) increases the expression of CD69 on the cell surface. The ability to detect the level of CD69 expression after TCR activation makes CD69 an ideal indicator of T cell activation (1). Alternate Names 5830438K24Rik; AI452015; AIM; Cd69; CD69 antigen; Early activation antigen CD69; VEA; Very Early Activation Antigen Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 40-55 Source/Isotype: Rabbit IgG