CST, 72298S, UCP1 (E9Z2V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UCP1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, UCP1 (E9Z2V) Rabbit Monoclonal Antibody (CST #72298) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (Frozen): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 60786 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity UCP1 (E9Z2V) Rabbit Monoclonal Antibody recognizes endogenous levels of total UCP1 protein. This antibody does not cross-react with UCP2 or UCP3 protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro152 of human UCP1 protein. Background Uncoupling protein 1 (UCP1) is a mitochondrial inner membrane transport protein that is primarily expressed in brown adipose tissue (BAT). UCP1 dissipates the pH gradient resulting from oxidative phosphorylation, which uncouples ATP synthesis from oxidative phosphorylation and leads to the release of heat energy. As a result, UCP1 plays an important role in thermogenesis (reviewed in 1). Research studies indicate that subcutaneous white adipose depots in mice contain beige adipocytes that express low levels of UCP1 protein (2). Additional studies show possible differences in thermogenesis in individuals carrying specific polymorphisms in the corresponding gene (3). Related studies link UCP1 to the possible development of obesity and type 2 diabetes (4). Alternate Names Mitochondrial brown fat uncoupling protein 1; SLC25A7; Solute carrier family 25 member 7; Thermogenin; UCP; UCP 1; UCP1; uncoupling protein 1; uncoupling protein 1 (mitochondrial, proton carrier) Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG