CST, 72312S, TSG101 (E6V1X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TSG101. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, TSG101 (E6V1X) Rabbit Monoclonal Antibody (CST #72312) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TSG101 (E6V1X) Rabbit Monoclonal Antibody recognizes endogenous levels of total TSG101 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TSG101 protein. Background Tumor susceptibility gene 101 protein (TSG101) is a multi-domain cytoplasmic protein and fundamental component of ESCRT complex I, which is intimately involved in regulating the trafficking of proteins throughout the endosomal compartment (1). In addition to regulating the sorting of ubiquitinated cargo proteins within the endosomal compartment, TSG101 is involved in regulating diverse biological processes, such as cell proliferation, viral budding and release, and exosome biosynthesis (2-6). While it is clear that TSG101 expression levels are tightly controlled and complete loss of expression causes embryonic lethality due to cell cycle arrest and cell death, the role of TSG101 in malignancy has yet to be fully elucidated (7). Indeed, TSG101 was initially dubbed a tumor suppressor but there are multiple studies that demonstrate an elevated expression in human cancers (8-10). Alternate Names ESCRT-I complex subunit TSG101; TS101; TSG10; TSG101; tumor susceptibility 101; tumor susceptibility gene 10; tumor susceptibility gene 101; Tumor susceptibility gene 101 protein; tumor susceptibility protein; VPS23 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG