CST, 72337T, TNF-R2 (E8D7P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TNF-R2. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, TNF-R2 (E8D7P) Rabbit Monoclonal Antibody (CST #72337) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 40409 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Live) Specificity / Sensitivity TNF-R2 (E8D7P) Rabbit Monoclonal Antibody recognizes endogenous levels of total TNF-R2 protein. This antibody cross-reacts with an unidentified protein of 180 kDa in some cell extracts. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human TNF-R2 protein. Background TNF-α is an important cytokine produced by numerous cell types, including neutrophils, activated lymphocytes, macrophages, and NK cells. It plays a critical role in inflammatory responses and apoptosis (1). TNF-α exists as a membrane-anchored and soluble form, both of which show biological activity. Response to TNF-α is mediated through two receptors, TNF-R1, which is widely expressed, and TNF-R2, which is expressed mainly in immune and endothelial cells (2). Antagonists to TNF-α have been validated as therapeutic targets for rheumatoid arthritis and other immune disorders (3). The two receptors for TNF-α, TNF-R1 (55 kDa) and TNF-R2 (75 kDa), can mediate distinct cellular responses (4,5). In most cases, cytotoxicity elicited by TNF has been reported to act through TNF-R1 (6,7). In contrast, TNF-R2 appears to be important in T cell signaling and responses to infection (7,8). TNF-R2 binds to distinct members of the TRAF family, leading to the activation of NF-κB (9,10). Soluble forms of both receptors have also been characterized which can bind TNF-α and may play an important role in immune disorders (11,12). Alternate Names CD120b; p75; p75 TNF receptor; p75TNFR; p80 TNF-alpha receptor; soluble TNFR1B variant 1; TBP-2; TBPII; TNF receptor superfamily member 1B; TNF-R-II; TNF-R2; TNF-R75; TNF-RII; TNFBR; TNFR-II; TNFR1B; TNFR2; TNFR80; TNFRSF1B; TNR1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor superfamily member 1b, membrane form; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II; Tumor necrosis factor-binding protein 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60-80 Source/Isotype: Rabbit IgG