CST, 72445S, GATA-2 (E8B5C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GATA2. Validated for WB,IP,IHC,IF,ChIP,ChIP,C&R,C&T. Available in 2 sizes. Highly specific and rigorously validated in-house, GATA-2 (E8B5C) Rabbit Monoclonal Antibody (CST #72445) is ready to ship. Product Usage Information For optimal ChIP and ChIP-Seq results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 . Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:2000 - 1:8000 Immunofluorescence (Immunocytochemistry): 1:800 - 1:1600 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 CUT&RUN: 1:50 CUT&Tag: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 67509 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Chromatin IP, Chromatin IP-seq, CUT&RUN, CUT&Tag Specificity / Sensitivity GATA-2 (E8B5C) Rabbit Monoclonal Antibody recognizes endogenous levels of total GATA-2 protein. Non-specific staining was observed in squamous epithelium by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly132 of human GATA-2 protein. Background The zinc finger transcription factor GATA-2 is widely expressed and plays an essential role in many developmental processes (1). Studies on GATA-2 knockout mice indicate that this protein is required in hematopoiesis (2). GATA-2 also inhibits the differentiation of white (3) and brown adipocytes (4) and has been shown to suppress the proliferation of neuronal progenitor cells (5). Alternate Names DCML; Endothelial transcription factor GATA-2; FLJ45948; GATA binding protein 2; GATA-binding protein 2; GATA2; IMD21; MGC2306; MONOMAC; NFE1B Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 51 Source/Isotype: Rabbit IgG