CST, 72604T, Stat2 (D9J7L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Stat2. Validated for WB,IP,IF,F,ChIP,ChIP,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, Stat2 (D9J7L) Rabbit Monoclonal Antibody (CST #72604) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 CUT&RUN: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 19413 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP, Chromatin IP-seq, CUT&RUN Specificity / Sensitivity Stat2 (D9J7L) Rabbit Monoclonal Antibody recognizes endogenous levels of total Stat2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu706 of human Stat2 protein. Background Stat2 (113 kDa), originally purified from the nuclei of alpha-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta (1,2). Knockout mice with a targeted disruption of Stat2 have higher susceptibility to viral infection and altered responses to type I interferons (3). Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via associations with receptor-bound Jak kinases (4). Unlike other Stat proteins, Stat2 does not form homodimers. Instead, activated Stat2 forms a heterodimer with Stat1 and translocates to the nucleus. There, it associates with the DNA-binding protein p48 and forms the transcriptional activator complex, interferon-stimulated gene factor 3 (ISGF3), promoting transcription from the ISRE (5). Alternate Names IMD44; interferon alpha induced transcriptional activator; ISGF-3; MGC59816; p113; PTORCH3; Signal transducer and activator of transcription 2; signal transducer and activator of transcription 2, 113kDa; STAT113; STAT2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 97, 113 Source/Isotype: Rabbit IgG