CST, 72610S, Tom20 (D8T4N) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying TOM20. Validated for Western Blotting. Highly specific and rigorously validated in-house, Tom20 (D8T4N) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #72610) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Tom20 (D8T4N) Rabbit Monoclonal Antibody (HRP Conjugate) recognizes endogenous levels of total Tom20 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Tom20 protein. Background Mitochondria play a central role in cellular energy metabolism and are essential organelles in eukaryotes. In humans, 13 proteins are encoded by the mitochondrial genome while the vast majority of mitochondrial proteins are encoded by the nuclear genome. As a result, most mitochondrial proteins are synthesized as precursors in the cytoplasm and imported across mitochondrial membranes by one or more translocase protein complexes (1). The translocase of the outer mitochondrial membrane (TOM complex) facilitates the import of proteins through the outer mitochondrial membrane, while the complementary translocase of the inner membrane (TIM complex) is responsible for protein transport to the mitochondrial matrix. The TOM complex consists of the receptors TOM20, TOM22, TOM70, and the channel-forming protein TOM40 (1). TOM20 is localized in the outer mitochondrial membrane and initially recognizes precursors with a presequence to facilitate protein import across the outer mitochondrial membrane (2). In a sequential process, recognition of the presequence by TOM20 is followed by tethering of the presequence to the TOM40 protein complex for efficient protein import (3). Alternate Names KIAA0016; MAS20; MGC117367; Mitochondrial 20 kDa outer membrane protein; Mitochondrial import receptor subunit TOM20 homolog; MOM19; Outer mitochondrial membrane receptor Tom20; TOM20; TOMM20; translocase of outer mitochondrial membrane 20; translocase of outer mitochondrial membrane 20 homolog (yeast); translocase of outer mitochondrial membrane 20 homolog type II Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 16 Source/Isotype: Rabbit IgG