CST, 7287V2, PathScan® Phospho-IRS-1 (Ser307) Sandwich ELISA Kit

ELISA Kit for studying IRS-1 (Ser312) phosphate in the research area. Protocol Available protocols: ELISA+ Specificity / Sensitivity PathScan Species Reactivity: Human, Mouse Background Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10). Alternate Names HIRS-1; Insulin receptor substrate 1; IRS-1; IRS1 Specification REACTIVITY: H M