CST, 72908T, PEAK1 (D4G6J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PEAK1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PEAK1 (D4G6J) Rabbit Monoclonal Antibody (CST #72908) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PEAK1 (D4G6J) Rabbit Monoclonal Antibody recognizes endogenous levels of total PEAK1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr695 of human PEAK1 protein. Background PEAK1 (Pseudopodium-enriched atypical kinase 1 or sgk269) is a member of nonreceptor atypical tyrosine kinase family identified by MS analysis of purified psedopodium (1). PEAK1 is a multi-domain protein with a N-terminal Erk binding site, followed by actin-targeting/Src substrate/Erk substrate region, Crk binding site, Shc binding site, and a C-terminal kinase domain (1, 2). By interacting with different adaptors like Shc, Grb2, Src, and others, PEAK1 functions as an important regulator in different signaling pathways, namely the Src/PEAK1/ErbB2 (3), EGFR Shc1/PEAK1/Grb2(4), TGFβ/PEAK1/Src/MAPK (5), and fibronectin/PEAK1/Src (6) pathways. PEAK1 plays an instrumental role in a wide variety of biological processes including epithelial-mesenchymal transition (EMT), dynamics of focal adhesion, cancer metastatic growth and invasion as well as cancer drug resistance (3, 5-8). Phosphorylation of PEAK1 at Tyr665 or Tyr635 by SFK (Src family Kinases) has been shown to be essential for cancer cell migration and invasion as well as the turnover of focal adhesions (7, 9). Alternate Names FLJ21140; FLJ34483; Inactive tyrosine-protein kinase PEAK1; KIAA2002; NKF3 kinase family member; PEAK1; pseudopodium enriched atypical kinase 1; Pseudopodium-enriched atypical kinase 1; SG269; SGK269; Sugen kinase 269; Tyrosine-protein kinase SgK269 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 250 Source/Isotype: Rabbit IgG