CST, 72943S, Fibronectin/FN1 (E5H6X) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying FN1. Validated for Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Fibronectin/FN1 (E5H6X) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #72943) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Fibronectin/FN1 (E5H6X) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a protein fragment corresponding to residues near the carboxy terminus of human Fibronectin/FN1 protein. Background Fibronectin is a multi-domain extracellular matrix (ECM) protein. The protein uses its different domains to bind distinct ECM components such as collagens, growth factors, and cell surface integrins to carry on its functions (1). Fibronectin has been implicated in many essential biological processes, including tissue repair, fibrosis, and tumor development (1,2). There are many fibronectin isoforms. Plasma fibronectin is synthesized by hepatocytes and exists as a compact, inactive conformation in the bloodstream. It is a major component of fibrin clots. Upon binding to integrins or other cell surface receptors, fibronectin switches to an extended conformation, and exposes its function domains to activated extracellular matrix assembly (3). Fibroblasts, endothelial cells, and many types of cancer cells have also been shown to synthesize cellular fibronectin isoforms. Among them, the EDA or EDB isoforms are particularly highly expressed during fibrosis and cancer development, and are potential diagnostic and therapeutic targets (4-6). Alternate Names Anastellin; CIG; Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED-B; epididymis secretory sperm binding protein; Fibronectin; fibronectin 1; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; migration-stimulating factor; MSF; SMDCF; Ugl-Y1; Ugl-Y2; Ugl-Y3 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG