CST, 72964S, LPCAT3 (E5G9P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MBOAT5. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, LPCAT3 (E5G9P) Rabbit Monoclonal Antibody (CST #72964) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LPCAT3 (E5G9P) Rabbit Monoclonal Antibody recognizes endogenous levels of total LPCAT3 protein. This antibody may detect nonspecific bands at 90 and 160 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys164 of human LPCAT3 protein. Background LPCAT3 (lysophosphatidylcholine acyltransferase 3), also called MBOAT5, is part of the LPCAT family that plays an important role in lipid metabolism (1,2). Its function is to catalyze the incorporation of fatty acids into phospholipids, lysophosphatidylcholine (lysoPC), lysophosphatidylserine (lysoPS), and lysophosphatidylethanolamine (lysoPE), to produce various fatty acid containing forms of PC, PS, and PE. It shows a preference for lysoPC and incorporation of linoleoyl and arachidonoyl chains as fatty acid donors (3,4). LPCAT3 is localized to the endoplasmic reticulum (ER) and is primarily expressed in metabolic tissues, including the liver, pancreas, and adipose tissue (2). It is a direct transcriptional target of LXR, PPARα, and PPARγ, which are lipid activated nuclear receptors that play important roles in regulating lipid homeostasis (2,5-7). Elevated expression of LPCAT3 is associated with the iron-dependent cell death process of ferroptosis that is characterized by an increase in lipid peroxidation (8). LPCAT3 inhibitors have shown a partial protection from ferroptosis (9). Dysregulation of LPCAT3 can have an important role in the development of diseases, including atherosclerosis, diabetes, intestinal tumors, and nonalcoholic steatohepatitis (NASH) (10,11). LPCAT3 is significantly upregulated in many cancers and is associated with prognosis (12). Alternate Names 1-acylglycerophosphocholine O-acyltransferase; 1-acylglycerophosphoethanolamine O-acyltransferase; 1-acylglycerophosphoserine O-acyltransferase; C3F; LPCAT; LPCAT3; LPLAT 5; LPSAT; Lyso-PC acyltransferase; Lyso-PC acyltransferase 3; Lyso-PS acyltransferase; Lysophosphatidylcholine acyltransferase; Lysophosphatidylcholine acyltransferase 3; Lysophosphatidylserine acyltransferase; Lysophospholipid acyltransferase 5; MBOA5; MBOAT5; membrane bound O-acyltransferase domain containing 5; Membrane-bound O-acyltransferase domain-containing protein 5; nessy; O-acyltransferase (membrane bound) domain containing 5; O-acyltransferase domain-containing protein 5; OACT5; putative protein similar to nessy Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 36 Source/Isotype: Rabbit IgG