CST, 73006SF, Rab11 (D4F5) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Rab11A/Rab11B. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Rab11 (D4F5) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #73006) is ready to ship. Product Usage Information This product is the carrier free version of product #5589. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 5589 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Rab11 (D4F5) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogeneous levels of Rab11 protein, including isoforms Rab11a and Rab11b. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Rab11 protein. Background Rab11a, Rab11b and Rab25 are members of the Rab11 family of small Ras-like GTPases. Rab11 (isoforms Rab11a and Rab11b) functions as a key regulator in the recycling of perinuclear, plasma membrane and Golgi compartment endosomes (1,2). Despite some overlap, distinct differences exist between Rab11a and Rab11b in both their cellular distribution and functional roles. Rab11a is ubiquitously expressed while Rab11b is found mainly in the heart and brain (3,4). Like other Rab proteins, Rab11 exerts its function via interactions with Rab11 family interacting proteins (FIPs). While there are three distinct classes of FIPs, all appear to share a conserved carboxy-terminal Rab-binding domain that allows Rab-FIP protein interaction. When bound together, these proteins are thought to regulate membrane-associated protein sorting (5,6). Alternate Names GTP-binding protein YPT3; H-YPT3; MGC133246; MGC1490; NDAGSCW; RAB 11A, member oncogene family; Rab-11; RAB11; RAB11A; RAB11A, member RAS oncogene family; RAB11B; RAB11B, member of RAS oncogene family; RAB11B, member RAS oncogene family; Ras-related protein Rab-11A; Ras-related protein Rab-11B; RB11A; RB11B; YL8; YPT3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG