CST, 73039S, MERS-CoV Nucleocapsid Protein Antibody

Polyclonal Antibody for studying Nucleocapsid Protein MERS-CoV. Validated for Western Blotting. Highly specific and rigorously validated in-house, MERS-CoV Nucleocapsid Protein Antibody (CST #73039) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MERS-CoV Nucleocapsid Protein Antibody recognizes transfected levels of total MERS-CoV nucleocapsid protein. Species Reactivity: Virus Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro382 of MERS-CoV nucleocapsid protein. Antibodies are purified by peptide affinity chromatography. Background Middle East Respiratory Syndrome (MERS) is a contagious viral respiratory infection, whose causative agent was identified as the MERS-related coronavirus (MERS-CoV) (1,2). MERS-CoV is a single-stranded mRNA betacoronavirus. Similar to other infectious coronaviruses (e.g., SARS-CoV-2), the MERS-CoV genome encodes four key structural proteins (spike, envelope, membrane, and nucleocapsid) that make up the virion particle, in addition to at least 10 smaller open reading frames (ORFs) that encode the non-structural (accessory) proteins involved in viral replication (3,4). Nucleocapsid protein is the primary protein involved in packaging the viral genome; however, its functions extend beyond its role in nucleocapsid formation. It may be translationally modified by host enzymes (5,6), and has been reported to influence viral transcription and replication, virion assembly, and the host response to viral infection, through effects on cell cycle, translation, and the innate immune response (7). Alternate Names MERS Nucleocapsid Protein; MERS-CoV nucleocapsid protein; NC; NCAP_CVEMC; nucleocapsid protein; Nucleoprotein; Protein N Specification REACTIVITY: Vir SENSITIVITY: Transfected Only MW (kDa): 55 SOURCE: Rabbit