CST, 73098T, GATAD2B (E4I8Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GATAD2B. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GATAD2B (E4I8Y) Rabbit Monoclonal Antibody (CST #73098) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GATAD2B (E4I8Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total GATAD2B protein. This antibody does not cross-react with GATAD2A protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val234 of human GATAD2B protein. Background GATAD2A and GATAD2B, also known as p66 alpha and p66 beta, are related members of the nucleosome remodeling and deacetylase (NuRD) complex (1). GATAD2B interacts with MBD2 to recruit the deacetylase core of the NuRD complex to repress target genes, a process that is enhanced by the sumoylation of GATAD2B at Lys33 (2-5). In addition to being sumoylated, GATAD2B has also been shown to be phosphorylated by Mkk6 during reprogramming (6). The GATAD2B protein contains an aggregation-prone region of its C-terminus which interacts with other NuRD members CHD4 and CDK2AP1 (7). Deletion of GATAD2B has been shown to be associated with severe intellectual disability (8,9). Alternate Names FLJ37346; GATA zinc finger domain containing 2B; GATA zinc finger domain-containing protein 2B; GATAD2B; KIAA1150; MGC138257; MGC138285; MRD18; p66/p68; P66B; P66beta; p68; RP11-216N14.6; transcription repressor p66 beta component of the MeCP1 complex; Transcriptional repressor p66-beta Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 77 Source/Isotype: Rabbit IgG