CST, 73152SF, STOP (175) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying MAP6 mouse. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, STOP (175) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #73152) is ready to ship. Product Usage Information This product is the carrier free version of product #4265. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 4265 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity STOP (175) Mouse Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total N-STOP and O-STOP proteins. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with purified N-STOP from rat brain. The antigen is encoded by exon 4 (Galiano et al., 2004). Background Stable Tubule Only Polypeptide (STOP) is a microtubule-associated protein, and its microtubule-stabilizing activity is regulated by calmodulin (1-2). STOPs have several tissue- and developmental-specific isoforms that are encoded by a single gene. Neurons express N-STOP (exons 1-4) and E-STOP (exons 1-3), fibroblasts express F-STOP (exons 1-2), oligodendrocytes express O-STOP, and astrocytes A-STOP (3). STOPs are the major contributors stabilizing microtubules that resist depolymerization due to cold or depolymerizing drugs. STOP knock-out mice display impaired synaptic plasticity associated with severe behavioral disorders in contrast to the anticipated neuronal development and brain anatomy defects (4). Alternate Names 2810411E12Rik; F-S; MAP-6; Map6; Microtubule-associated protein 6; Mta; Mtap6; Stable tubule-only polypeptide; STOP Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Mouse IgG1