CST, 73204SF, Cyclin C (E6V4Z) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Cyclin C. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Cyclin C (E6V4Z) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #73204) is ready to ship. Product Usage Information This product is the carrier free version of product #68179. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 68179 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Cyclin C (E6V4Z) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total cyclin C protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro263 of human cyclin C protein. Background Cyclin dependent kinases (CDKs) are regulated in part through binding to regulatory cyclins. While many cyclin/CDK complexes regulate progression through the cell cycle, others, such as cyclin C/CDK8, regulate transcription and other cellular functions. Cyclin C/CDK8 forms a regulatory sub-complex with Med12 and Med13, which associates with the multi-subunit transcriptional coactivator Mediator Complex to modulate RNA polymerase II-dependent transcription. In addition to its role in transcription, cyclin C is important in the regulation of mitochondrial dynamics, apoptosis, and cell cycle progression (1). Cytosolic cyclin C induces mitochondrial fragmentation and apoptosis, and is required for activation of the Bcl2 family member, Bax (2). Cytoplasmic cyclin C also supports mitochondrial fission through dynamin-related protein 1 (Drp1) in response to oxidative stress (3). Cyclin C acts synergistically with PTEN to maintain levels of tumor suppressors p53 and p21 in a mouse model of thyroid cancer (4). In complex with CDK3, cyclin C regulates Rb-dependent exit from quiescence in human cells (5). Alternate Names CCNC; CycC; cyclin C; Cyclin-C; hSRB11; SRB11; SRB11 homolog Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG