CST, 73216W2, NKX2.5 (E1Y8H) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 700 Conjugate)

Monoclonal Antibody for studying NKX2-5. Highly specific and rigorously validated in-house, NKX2.5 (E1Y8H) Rabbit Monoclonal Antibody (SignalFlex^™ Alexa Fluor^® 700 Conjugate) (CST #73216) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity NKX2.5 (E1Y8H) Rabbit mAb (SignalFlex™ Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro67 of human NKX2.5 protein. Background NKX2.5 is a member of the NKX homeobox transcription factor family. NKX2.5 plays an essential role in heart development and is among the earliest factors expressed in the cardiac lineage in developing embryos. Targeted disruption of the murine gene results in abnormal heart morphogenesis, severe growth retardation, and embryonic lethality around E9.5 (1,2). Mutations in are likewise associated with several congenital heart conditions, such as atrial defect with atrioventricular conduction defects (ASD-AVCD) and Tetralogy of Fallot (TOF) (3,4). Transcriptional activation of is also associated with some B and T cell leukemias that result from chromosomal translocation (5-8). Alternate Names Cardiac-specific homeobox; cardiac-specific homeobox 1; CHNG5; CSX; CSX1; FLJ52202; FLJ97166; FLJ97195; FLJ97197; FLJ99536; HLHS2; Homeobox protein CSX; Homeobox protein NK-2 homolog E; homeobox protein NKX 2-5; Homeobox protein Nkx-2.5; NK2 homeobox 5; NK2 transcription factor related, locus 5; NK2 transcription factor related, locus 5 (Drosophila); NKX 2-5; NKX2-5; NKX2.5; NKX25; NKX2E; NKX4-1; tinman homolog; tinman paralog; VSD3 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG