CST, 73225S, GLTSCR2 Antibody

Polyclonal Antibody for studying GLTSCR2. Validated for Western Blotting. Highly specific and rigorously validated in-house, GLTSCR2 Antibody (CST #73225) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GLTSCR2 Antibody recognizes endogenous levels of total GLTSCR2 protein. . Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys410 of human GLTSCR2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Glioma tumor suppressor candidate region gene 2 protein (GLTSCR2), also known as protein interacting with carboxyl terminus-1 (PICT-1), displays tumor suppressor activity, in part, by interacting with the C-terminal region of PTEN and preventing its degradation. Disruption of this interaction upregulates activity of the PI3K/Akt signaling axis and promotes cell transformation (1,2). Further evidence to support the tumor suppressor role of GLTSCR2 lies in the finding that is localized to the tumor suppressive region of chromosome 19q, which is subject to lesions in human brain tumors (3). Indeed, research studies have demonstrated that harbors nonsense mutations and deletions in glioblastomas, which lead to a decrease in GLTSCR2 protein expression (4). GLTSCR2 has also been shown to exert tumor suppressor activity through its involvement in the nucleolar stress response. Research studies indicate that in response to ribosomal stress, GLTSCR2 translocates from the nucleolus to the nucleoplasm where it binds and stabilizes p53 tumor suppressor, which results in inhibition of cell cycle progression (5). Alternate Names glioma tumor suppressor candidate region gene 2; Glioma tumor suppressor candidate region gene 2 protein; GLT; GLTSCR2; GSCR2; NOP53; NOP53 ribosome biogenesis factor; p60; PICT-1; PICT1; protein interacting with carboxyl terminus 1; Ribosome biogenesis protein NOP53 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 62 SOURCE: Rabbit