CST, 73235S, ISG20 Antibody

Polyclonal Antibody for studying ISG20. Validated for Western Blotting. Highly specific and rigorously validated in-house, ISG20 Antibody (CST #73235) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ISG20 Antibody recognizes endogenous levels of total ISG20 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ISG20 protein. Antibodies are purified by peptide affinity chromatography. Background Interferon-stimulated 20 kDa protein (ISG20) is an exoribonuclease that functions as part of the host innate immune response to RNA-based viruses. Indeed, expression of ISG20 can be induced by type-I and type-II interferons following cellular exposure to RNA-based viruses (1). Mechanistically, ISG20 localizes to the nucleolus and is believed to inhibit the replication of RNA-based viruses, in part, by employing 3'-5' exonuclease activity toward viral RNAs (2-5). Alternate Names CD25; Estrogen-regulated transcript 45 protein; HEM45; interferon stimulated exonuclease gene 20; interferon stimulated exonuclease gene 20kDa; Interferon-stimulated gene 20 kDa protein; ISG20; Promyelocytic leukemia nuclear body-associated protein ISG20 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 20 SOURCE: Rabbit