CST, 73236SF, MLANA/MART-1 (E9Q4O) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying MLANA. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, MLANA/MART-1 (E9Q4O) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #73236) is ready to ship. Product Usage Information This product is the carrier free version of product #64718. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 64718 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity MLANA/MART-1 (E9Q4O) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total MLANA/MART-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MLANA/MART-1 protein. Background MLANA, also known as MART-1, is a member of a melanocyte lineage-specific family of proteins. It is expressed in melanocytes, retinal pigment epithelium, and melanoma cells. Its function is not entirely understood, but it is believed to be involved in the stability of GPR143, as well as the stability, trafficking, and processing of PMEL; both proteins are involved in the formation of stage II melanosomes (1). In melanosomes, MLANA is specifically located in the trans-Golgi network, however conformational changes to the protein or a sub-population of the protein causes it to localize back to the ER and small endosomal vesicles (2). In the context of melanoma cells, the conformational change is thought to be caused by aberrant exposure of epitopes, which are recognized by cytolytic T-lymphocytes (3). MLANA may be useful as a marker of metastatic melanoma (4). MHC-II restricted phospho-MLANA peptides, which are recognized by CD4 cells, are being investigated as potential candidates for cancer immunotherapy (5). Alternate Names Antigen LB39-AA; Antigen SK29-AA; epididymis secretory sperm binding protein; MAR1; MART-1; MART1; melan-A; Melanoma antigen recognized by T-cells 1; MLANA; Protein Melan-A Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 19 Source/Isotype: Rabbit IgG