CST, 73239SF, EAAT2 (E3P5K) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying EAAT2. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, EAAT2 (E3P5K) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #73239) is ready to ship. Product Usage Information This product is the carrier free version of product #20848. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 20848 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity EAAT2 (E3P5K) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total EAAT2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human EAAT2 protein. Background Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. During neurotransmission, glutamate is released from vesicles of the pre-synaptic cell, and glutamate receptors (e.g., NMDA Receptor, AMPA Receptor) bind glutamate for activation at the opposing post-synaptic cell. Excitatory amino acid transporters (EAATs) regulate and maintain extracellular glutamate concentrations below excitotoxic levels. In addition, glutamate transporters may limit the duration of synaptic excitation by an electrogenic process in which the transmitter is cotransported with three sodium ions and one proton, followed by countertransport of a potassium ion. Five EAATs (EAAT1-5) are characterized: EAAT2 (GLT-1) is primarily expressed in astrocytes but is also expressed in neurons of the retina and during fetal development (1). Homozygous EAAT2 knockout mice have spontaneous, lethal seizures and an increased predisposition to acute cortical injury (2). PKC phosphorylates Ser113 of EAAT2 and coincides with glutamate transport (3). Alternate Names EAA2; EAAT2; EIEE41; Excitatory amino acid transporter 2; excitotoxic amino acid transporter 2; glial high affinity glutamate transporter; GLT-1; GLT1; Glutamate/aspartate transporter II; HBGT; SLC1A2; Sodium-dependent glutamate/aspartate transporter 2; solute carrier family 1 (glial high affinity glutamate transporter), member 2; Solute carrier family 1 member 2; solute carrier family 1, member 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG