CST, 73257W2, IkappaB alpha (L35A5) Mouse Monoclonal Antibody (Amino-terminal Antigen) (SignalFlex™ Alexa Fluor® 750 Conjugate)

Monoclonal Antibody for studying IkB-a. Highly specific and rigorously validated in-house, IkappaB alpha (L35A5) Mouse Monoclonal Antibody (Amino-terminal Antigen) (SignalFlex^™ Alexa Fluor^® 750 Conjugate) (CST #73257) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (SignalFlex™ Alexa Fluor Species Reactivity: Human, Mouse, Rat, Monkey, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a GST-IκBα fusion protein corresponding to the amino terminus of human IκBα. Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). Alternate Names EDAID2; I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; MAD-3; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; NFKB inhibitor alpha; NFKBI; NFKBIA; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha Specification REACTIVITY: H M R Mk B Pg SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1