CST, 7327C, PathScan® Phospho-PRAS40 (Thr246) Sandwich ELISA Kit

ELISA Kit for studying PRAS40 (Thr246) phosphate in the research area. Protocol Available protocols: ELISA+ Specificity / Sensitivity PathScan Species Reactivity: Human, Mouse Background Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5). Alternate Names 40 kDa proline-rich AKT substrate; AKT1 substrate 1; AKT1 substrate 1 (proline rich); AKT1S1; AKTS1; Lobe; PRAS40; Proline-rich AKT1 substrate 1 Specification REACTIVITY: H M