CST, 73288S, c-Myb (D1B9E) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying c-Myb. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, c-Myb (D1B9E) Rabbit Monoclonal Antibody (PE Conjugate) (CST #73288) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity c-Myb (D1B9E) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total c-Myb protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human c-Myb protein. Background c-Myb is a transcriptional activator that specifically recognizes the sequence 5'-YAAC[GT]G-3'. It is expressed in hematopoietic progenitor cells, where it plays an important role in the control of proliferation and differentiation (1-3). c-Myb is required for transcription of genes involved in self-renewal of intestinal stem cells. Importantly, c-Myb regulates expression of Lgr5, a protein expressed in putative intestinal stem cells that give rise to all cell lineages of small intestinal crypts (4). c-Myb is reported to be expressed in colon crypt cells and in human colorectal cancer cell lines (5,6). Research has shown that gene translocations and copy number alterations are found in several leukemias, breast cancer, and other solid tumors (7,8). Alternate Names c-myb; c-myb protein (140 AA); c-myb_CDS; c-myb10A_CDS; c-myb13A_CDS; c-myb14A_CDS; c-myb8B_CDS; Cmyb; efg; MYB; MYB proto-oncogene, transcription factor; oncogene AMV; Proto-oncogene c-Myb; Transcriptional activator Myb; v-myb avian myeloblastosis viral oncogene homolog; v-myb myeloblastosis viral oncogene homolog (avian) Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG