CST, 73294S, LAMP1 (E5N9Z) Rabbit Monoclonal Antibody (Alexa Fluor® 594 Conjugate)

Monoclonal Antibody for studying LAMP1 mouse. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, LAMP1 (E5N9Z) Rabbit Monoclonal Antibody (Alexa Fluor^® 594 Conjugate) (CST #73294) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:100 - 1:200 Immunofluorescence (Immunocytochemistry): 1:400 - 1:800 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity LAMP1 (E5N9Z) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse LAMP1 protein. Background Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6). Alternate Names 120 kDa lysosomal membrane glycoprotein; AI196048; CD107; CD107 antigen-like family member A; CD107a; Lam; Lamp I; LAMP-1; Lamp1; LGP-120; LGP-A; lysosomal membrane glycoprotein 1; Lysosomal membrane glycoprotein A; lysosomal-associated membrane protein 1; Lysosome-associated membrane glycoprotein 1; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1 PRECURSOR (LAMP-1) (LGP-A) (LGP-120) (CD107A) (P2B); Lysosome-associated membrane protein 1; P2B; Perk Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG