CST, 73317SF, Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying MKK3 (Ser218) phosphate/MKK6 (Ser207) phosphate. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #73317) is ready to ship. Product Usage Information This product is the carrier free version of product #12280. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12280 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of MKK3 and MKK6 proteins only when phosphorylated at Ser189 (MKK3) or Ser207 (MKK6). Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser189 of human MKK3 protein and Ser207 of human MKK6 protein. Background MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation at Ser189 and Thr222 on MKK3 (2) and Ser207 and Thr211 on MKK6 (4,5). Alternate Names Dual specificity mitogen-activated protein kinase kinase 3; Dual specificity mitogen-activated protein kinase kinase 6; MAP kinase kinase 3; MAP kinase kinase 6; MAP2K3; MAP2K6; MAPK/ERK kinase 3; MAPK/ERK kinase 6; MAPKK 3; MAPKK 6; MAPKK3; MAPKK6; MEK 3; MEK 6; MEK3; MEK6; mitogen-activated protein kinase kinase 3; mitogen-activated protein kinase kinase 6; MKK3; MKK6; MP2K3; MP2K6; PRKMK3; PRKMK6; protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6); SAPK kinase 2; SAPK kinase 3; SAPKK-2; SAPKK-3; SAPKK2; SAPKK3; SKK2; SKK3; Stress-activated protein kinase kinase 2; Stress-activated protein kinase kinase 3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 38 MKK6, 40 MKK3 Source/Isotype: Rabbit IgG