CST, 73348S, Desmin (F5V4I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Desmin. Validated for Western Blotting,Simple Western™,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Desmin (F5V4I) Rabbit Monoclonal Antibody (CST #73348) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 62742 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Desmin (F5V4I) Rabbit Monoclonal Antibody recognizes endogenous levels of total desmin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg429 of human desmin protein. Background The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are distinguished and expressed in particular cell types: cytokeratins (epithelial cells), glial fibrillary acidic protein or GFAP (glial cells), desmin (skeletal, visceral, and certain vascular smooth muscle cells), vimentin (mesenchyme origin), and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). Vimentin is present in sarcomas but not carcinomas, and its expression is examined relative to other markers to distinguish between the two forms of neoplasm (3). Desmin is a myogenic marker expressed in early development that forms a network of filaments that extends across the myofibril and surrounds Z discs. The desmin cytoskeleton provides a connection among myofibrils, organelles, and the cytoskeleton (4). Desmin knockout mice develop cardiomyopathy and skeletal and smooth muscle defects (5). In humans, desmin-related myopathies might be caused by mutations in the corresponding desmin gene or in proteins with which desmin interacts, including αB-crystallin and synemin. Disorganized desmin filaments and the accumulation of protein aggregates composed predominantly of desmin characterize desmin-related myopathies (reviewed in 6,7). Alternate Names cardiomyopathy, dilated 1F (autosomal dominant); CDCD3; CMD1I; CSM1; CSM2; DES; DESM; Desmin; epididymis secretory sperm binding protein; FLJ12025; FLJ39719; FLJ41013; FLJ41793; intermediate filament protein; LGMD1D; LGMD1E; LGMD2R Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 53 Source/Isotype: Rabbit IgG