CST, 73425S, S100A9 (D3U8M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying S100A9 mouse. Validated for WB,IP,IHC,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, S100A9 (D3U8M) Rabbit Monoclonal Antibody (CST #73425) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 IHC Leica Bond: 1:800 Immunohistochemistry (Paraffin): 1:800 Immunofluorescence (Frozen): 1:100 Flow Cytometry (Fixed/Permeabilized): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 74641 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity S100A9 (D3U8M) Rabbit Monoclonal Antibody recognizes endogenous levels of total S100A9 protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn98 of mouse S100A9 protein. Background S100A8 and S100A9 are calcium-binding proteins that form a noncovalent heterodimer present in monocytes, neutrophils, macrophages, and some epithelial cells (1,2). S100A8 and S100A9 are secreted by a tubulin-dependent mechanism during inflammatory conditions and have antimicrobial and chemotactic functions (3-5). Extracellular S100A8/S100A9 also induces an inflammatory response in endothelial cells, including induction of proinflammatory chemokines and adhesion molecules and increased vascular permeability (6). S100A8/S100A9 induces and recruits myeloid-derived suppressor cells (MDSC) in tumor-bearing mice (7). MDSC produce additional S100A8/S100A9 themselves, resulting in a positive feedback mechanism that sustains MDSC accumulation (7). S100A8/S100A9 is also highly expressed in psoriatic skin, where it directly upregulates transcription of complement protein C3, which contributes to disease (8). In addition, tumor-infiltrating myeloid cells induce expression of S100A8 and S100A9 in cancer cells, which increases invasiveness and metastasis (9). Alternate Names 60B8A; 60B8Ag; AW546964; BEE22; Cag; Cagb; Calgranulin-B; GAG; GAGB; L1Ag; Leukocyte L1 complex heavy chain; Migration inhibitory factor-related protein 14; MRP; MRP-14; Mrp14; p14; Protein S100-A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100a9; S10A9 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 14 Source/Isotype: Rabbit IgG