CST, 73540T, VPS29 (D2H7G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VPS29. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, VPS29 (D2H7G) Rabbit Monoclonal Antibody (CST #73540) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity VPS29 (D2H7G) Rabbit Monoclonal Antibody recognizes endogenous levels of total VPS29 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala121 of human VPS29 protein. Background Retromer is a heteropentameric protein complex that consists of two protein compounds, a sortin-nexin dimer and a trimeric VPS26-VPS29-VPS35 protein subcomplex. The retromer complex associates with endosomes at their cytosolic side to mediate retrograde transport of transmembrane proteins from endosomes to the trans-Golgi network (1-3). Vacuolar protein sorting-associated protein 29 (VPS29) is considered a cryptic metallophosphoesterase, as it contains a conserved metallophosphoesterase-fold that includes a phenylalanine in place of an essential histidine residue within the active site (4). While VPS29 is capable of binding metal ions, it does so with low affinity and exhibits no enzymatic activity. Instead, VPS29 serves as a scaffold protein that interacts with the carboxy-terminal region of VPS35 and is essential for association of the retromer with other endosomal transport proteins (5). Alternate Names DC15; DC7; DKFZp564F0223; epididymis secretory sperm binding protein; FLJ20492; hVPS29; PEP11; PEP11 homolog; retromer protein; vacuolar protein sorting 29 homolog; vacuolar protein sorting 29 homolog (S. cerevisiae); Vacuolar protein sorting-associated protein 29; vacuolar sorting protein VPS29/PEP11; Vesicle protein sorting 29; VPS29; VPS29 retromer complex component; x 007 protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 21 Source/Isotype: Rabbit IgG