CST, 73583SF, SIX1 (D5S2S) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SIX1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, SIX1 (D5S2S) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #73583) is ready to ship. Product Usage Information This product is the carrier free version of product #16960. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 16960 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity SIX1 (D5S2S) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total SIX1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp269 of human SIX1 protein. Background Sine oculis homeobox (SIX) proteins belong to a family of evolutionarily conserved transcription factors discovered in mutant screens for embryonic eye development genes (1-3). The prototypical family member ( , ) was named for eyeless embryos carrying mutations in a gene highly conserved among vertebrates, including humans ( ) (4). A total of six family members (SIX1-6) have been identified in vertebrates. Each SIX protein contains a homeobox nucleic acid recognition domain (HD) with a DNA-binding helix-turn-helix motif and an adjacent SIX domain, which may be involved in regulating protein-protein interactions (5). In addition to their critical functions during embryonic organogenesis, research studies suggest that SIX proteins play additional roles in postnatal cell cycle regulation, with potentially important implications in tumorigenesis (6,7). In contrast to the ortholog, the vertebrate gene product does not play a critical role in embryonic eye development. Vertebrate SIX1 is required for development of mesoderm- and neural crest-derived lineages, and male reproductive tissues (8-10). SIX1 has also been shown to regulate transcription of MyoD in adult muscle progenitor cells during postnatal muscle development (11). A mechanistic role for SIX1 in cell cycle regulation is supported by research studies showing increased SIX1 expression in various cancer subtypes, including breast, ovarian, and hepatocellular carcinoma (6,12,13). Alternate Names BOS3; DFNA23; Homeobox protein SIX1; Sine oculis homeobox homolog 1; SIX homeobox 1; SIX1; TIP39 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 36 Source/Isotype: Rabbit IgG