CST, 73758S, Tox/Tox2 (E6I3Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Tox2/Tox. Validated for WB,IP,IHC,IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, Tox/Tox2 (E6I3Q) Rabbit Monoclonal Antibody (CST #73758) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 IHC Leica Bond: 1:800 - 1:3200 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 62886 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity Tox/Tox2 (E6I3Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total Tox and Tox2 proteins. This antibody does not cross-react with Tox3 or Tox4 proteins. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala522 of human Tox protein. Background Thymocyte selection-associated high mobility group box protein (Tox) is a DNA-binding nuclear factor and member of the evolutionarily conserved high-motility group (HMG)-box superfamily. Tox also defines a small subfamily of proteins that include Tox2, Tox3, and Tox4, all of which are highly conserved in vertebrate species but have unique tissue expression patterns and functions (1,2). Tox plays a key role in T cell development in the thymus during positive selection (3-5). A study in Tox-deficient mice also revealed a requirement for Tox in CD4 T cell and NK cell lineage development, including NKT cells, FoxP3 T regulatory T cells, and lymphoid tissue-inducer (LTi) cells (6-8). Although Tox expression is primarily restricted to developing immune cells in normal tissues, Tox is induced by high antigen stimulation during chronic viral infection or cancer, regulating T cell persistence and exhaustion (9-12). Tox has also been shown to be aberrantly expressed in cutaneous T cell lymphomas (13-14). Alternate Names C20orf100; dJ1108D11.2; dJ495O3.1; GCX-1; GCX1; granulosa cell HMG box 1; Granulosa cell HMG box protein 1; granulosa cell HMG-box protein 1; KIAA0808; MGC15880; thymocyte selection associated high mobility group box; thymocyte selection-associated high mobility group box; Thymocyte selection-associated high mobility group box protein TOX; Thymus high mobility group box protein TOX; TOX; TOX high mobility group box family member 2; TOX1; TOX2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 60-80 Source/Isotype: Rabbit IgG