CST, 73799SF, Myelin Basic Protein (2H9) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying MBP. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, Myelin Basic Protein (2H9) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #73799) is ready to ship. Product Usage Information This product is the carrier free version of product #13344. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13344 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Myelin Basic Protein (2H9) Mouse Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total myelin basic protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human myelin basic protein. Background Myelin basic protein (MBP) is an abundant central nervous system (CNS) myelin membrane protein that plays an important role in nerve myelination. Myelin sheaths are multi-layered membranes derived from oligodendrocytes that increase the conduction velocity of axonal impulses. MBP helps to adhere the cytoplasmic leaflets of adjacent oligodendrocyte membranes to one another. Several splice variants of MBP are expressed in cells and the protein is modified following translation (i.e., phosphorylation, methylation), which suggests additional membrane adhesion functions (1). Alternate Names Golli-MBP; MBP; MGC99675; Myelin A1 protein; Myelin basic protein; Myelin membrane encephalitogenic protein; proline-rich proteoglycan 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 12-18 Source/Isotype: Mouse IgG1