CST, 73812S, Hydroxyproline Antibody

Polyclonal Antibody for studying . Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Hydroxyproline Antibody (CST #73812) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity Hydroxyproline Antibody recognizes endogenous levels of proline only when the proline is hydroxylated. This antibody does not react with non-hydroxylated proline. Species Reactivity: All Species Expected Source / Purification Polyclonal antibodies are produced by immunizing animals with an antigen containing hydroxyproline. Antibodies are purified by affinity chromatography. Background Collagens are a large family of proteins and collectively they are the most abundant protein in mammals. They are trimeric molecules comprised of three alpha polypeptide chains that form a triple helix structure that is characteristic of all collagens (1). Collagen chains have important features that allow helix to form: every third amino acid in the sequence is a glycine, and the chains are co- and post-translationally modified. Prolines are hydroxylated by collagen prolyl 4-hydroxylases. These hydroxyproline modifications are critical for the stability of the triple helix to form fibrils (2). While proline hydroxylation occurs on a small number of other proteins, the hydroxyproline content of tissue comes almost entirely from collagen, such that hydroxyproline quantitative biochemical assays are routinely utilized as a measurement of collagen (3). Specification REACTIVITY: All SENSITIVITY: Endogenous SOURCE: Rabbit