CST, 73828T, INO80 (F2I3E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying INOC1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, INO80 (F2I3E) Rabbit Monoclonal Antibody (CST #73828) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity INO80 (F2I3E) Rabbit Monoclonal Antibody recognizes endogenous levels of total INO80 protein. This antibody detects bands of unknown origin at 145, 55, and 50 kDa. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1547 of human INO80 protein. Background The INO80 complex is an ATP-dependent chromatin remodeling complex critical for many functions, including transcriptional regulation and DNA repair. INO80 is a large multi-subunit complex consisting of the key ATPase INO80 along with RUVBL1, RUVBL2, ARP4/5/8, and others (1-3). INO80 prefers binding to hexasomes and can facilitate the exchange of H2A.Z/H2B dimers with H2A/H2B (4-6). The INO80 complex is recruited to double-strand breaks (DSBs) through interaction between gamma-H2AX and complex member NHP10 (7,8). INO80 also plays an important role in transcription as it is required for proper pausing of RNA polymerase II (9). Alternate Names Chromatin-remodeling ATPase INO80; DNA helicase INO80; DNA helicase-related INO80 complex homolog 1; DNA helicase-related protein INO80; hINO80; homolog of yeast INO80; INO80; INO80 complex ATPase subunit; INO80 complex homolog 1; INO80 complex subunit A; INO80 homolog (S. cerevisiae); INO80A; INOC1; KIAA1259; Putative DNA helicase INO80 complex homolog 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 280 Source/Isotype: Rabbit IgG