CST, 73842S, Phospho-PDGF Receptor alpha/beta (panTyr) Matched Antibody Pair

Matched Antibody Pair for studying PDGFR-a (Tyr) phosphate/PDGFR-b (Tyr) phosphate in the research area. Product Usage Information Matched Antibody Pairs consist of capture and detection antibodies that bind to non-overlapping epitopes. For specific identification of the capture and detection antibodies in this pair, please refer to the data figure caption. Optimal dilutions/concentrations should be determined by the end user. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. Storage Store at -20ºC. This product will freeze at -20ºC so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles . A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Background Platelet-derived growth factor (PDGF) is a dimeric molecule that exists in homodimers or heterodimers of related polypeptide chains (A and B). Two types of PDGF receptors have been identified. The PDGF alpha-receptor binds all three PDGF isoforms with high affinity, whereas the beta-receptor binds only PDGF-BB with high affinity, PDGF-AB with low affinity, and does not appear to bind PDGF-AA (1). Ligand binding induces receptor dimerization and autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules. A number of different signaling pathways are then initiated, leading to cell growth, actin reorganization, migration, and differentiation (2-4). In clinical studies, PDGF expression has been demonstrated in a number of different solid tumors, from glioblastomas to prostate carcinomas. In these various tumor types, the biological role of PDGF signaling can vary from autocrine stimulation of cancer cell growth to more subtle paracrine interactions involving adjacent stroma and even angiogenesis. Targeting PDGF signaling is becoming an effective tumor treatment method (5). Alternate Names Activated tyrosine kinase PDGFRB; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140; CD140 antigen-like family member A; CD140 antigen-like family member B; CD140A; CD140a antigen; CD140B; IBGC4; IMF1; JTK12; KOGS; MGC74795; NDEL1-PDGFRB; PDGF-R-alpha; PDGF-R-beta; PDGFR; PDGFR alpha; PDGFR beta; PDGFR-1; PDGFR-2; PDGFR-alpha; PDGFR-beta; PDGFR1; PDGFR2; PDGFRA; PDGFRalpha; PDGFRB; PENTT; PGFRA; PGFRB; platelet derived growth factor receptor alpha; platelet derived growth factor receptor beta; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor 2; Platelet-derived growth factor receptor alpha; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor, alpha polypeptide; platelet-derived growth factor receptor, beta polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alpha fusion protein; RHEPDGFRA Specification REACTIVITY: H M