CST, 73901S, PP2

Chemical Modulators for studying in the research area. Product Usage Information PP2 is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 5 mg of powder in 1.1 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect. Storage Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles. Background PP2 is a potent and selective inhibitor of Src-family kinases. This small molecule inhibits Lck (IC = 4 nM), Fyn (IC = 5 nM), and Hck (IC = 5 nM) with little to no effect on ZAP-70 (IC > 100 µM) and JAK2 (IC > 50 µM) (1). Members of the Src family are involved in the regulation of cellular excitability and synaptic plasticity. Inhibition of Src family nonreceptor protein tyrosine kinases (PTKs) can decrease epileptiform activity, making PP2 an important compound when studying epilepsy and antiepileptic therapies (2). Alternate Names 1-tert-Butyl-3-4-chlorophenyl-1H-pyrazolo[34-d]pyrimidin-4-amine; 3-4-chlorophenyl-1-11-dimethylethyl-1H-pyrazolo[34-d]pyrimidin-4-amine; 4-Amino-3-4-chlorophenyl-1-t-butyl-1H-pyrazolo[34-d]pyrimidine; 4-Amino-5-4-chlorophenyl-7-t-butylpyrazolo[34-d]pyrimidine; AG 1879; AGL 1879; PP1