CST, 73930S, CTHRC1 (E8M9S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CTHRC1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CTHRC1 (E8M9S) Rabbit Monoclonal Antibody (CST #73930) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity CTHRC1 (E8M9S) Rabbit Monoclonal Antibody recognizes endogenous levels of total CTHRC1 protein. Non-specific staining was observed in human pancreatic islets and in mouse squamous epithelium and mucin by immunohistochemistry. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CTHRC1 protein. Background Collagen triple helix repeat containing-1 (CTHRC1) is a secreted glycoprotein involved in wound repair, vascular and bone remodeling, matrix deposition, and migration. CTHRC1 is overexpressed in multiple human cancers, and its expression is associated with increased migration and metastasis, as well as decreased survival (1-3). CTHRC1 expression is regulated through TGF-β/Smad3 signaling, and its effects on migration and invasion are mediated by several signaling pathways, including TGF-β, Wnt, integrin β/FAK, Src/FAK, MEK/ERK, PI3K/AKT/ERK, HIF-1α, and PKC-δ/ERK (3). CTHRC1 protein is elevated in the peripheral blood of Rheumatoid arthritis (RA) patients, and CTHRC1 is thought to be a driver of RA progression (4). Alternate Names collagen triple helix repeat containing 1; Collagen triple helix repeat-containing protein 1; CTHR1; CTHRC1; Q96CG8 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rabbit IgG