CST, 73989T, MPEG1 (F1S7U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MPEG1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MPEG1 (F1S7U) Rabbit Monoclonal Antibody (CST #73989) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MPEG1 (F1S7U) Rabbit Monoclonal Antibody recognizes endogenous levels of total MPEG1 protein. This antibody detects a 28 kDa and an 180 kDa protein of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly550 of human MPEG1 protein. Background Macrophage-expressed gene 1 protein (MPEG1), also known as perforin-2, is a member of the Membrane Attack Complex/Perforin (MACPF) superfamily of pore-forming proteins (1,4). MPEG1, mainly expressed in macrophages and leukocytes, can be upregulated in response to proinflammatory signals such as tumor necrosis factor α (TNF-α) and lipopolysaccharides (LPS) (1-3). MPEG1 is involved in both innate and adaptive immunity, localizing to early endosomes and phagosomes/lysosomes, and acts in an antimicrobial capacity (4). In dendritic cells, MPEG1 helps deliver antigens into the cytosol, where they can be processed and presented on MHC class I molecules to activate CD8 T cells (5). Alternate Names likely ortholog of mouse macrophage expressed gene 1; macrophage expressed 1; macrophage expressed gene 1; Macrophage gene 1 protein; Macrophage-expressed gene 1 protein; MGC132657; MGC138435; MPEG1; Mpg-1; MPG1; MPS1; P-2; partial protein; perforin 2; Perforin-2; Q2M385 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80-100 Source/Isotype: Rabbit IgG