CST, 74030T, NUFIP1 (E7E4L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NUFIP1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NUFIP1 (E7E4L) Rabbit Monoclonal Antibody (CST #74030) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NUFIP1 (E7E4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total NUFIP1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NUFIP1 protein. Background NUFIP1 (nuclear FMRP interacting protein 1) was identified as an RNA-binding protein shuttling between the nucleus and cytoplasm, and it associates with ribosomes in functional synaptoneurosomes (1). Reports have shown that NUFIP1 can function as a receptor for starvation-induced ribophagy to maintain protein homeostasis (2). Ribophagy is a selective form of autophagy targeting ribosomes (reviewed in 3). Lysosomal levels of NUFIP1 were increased upon inhibition of autophagy. Ribophagy was dependent on the ability of ribosome-associated NUFIP1 to interact with LC3B via a conserved LC3-interacting region (LIR) domain and recruit ribosomes to the autophagosome (2). Loss of NUFIP1 or its LC3B binding ability inhibited cell survival following starvation (2). Alternate Names bA540M5.1; FMR1-interacting protein NUFIP1; nuclear FMR1 interacting protein 1; Nuclear FMRP-interacting protein 1; nuclear fragile X mental retardation protein interacting protein 1; nuclear fragile X mental retardation protein-interacting protein 1; Nuclear fragile X mental retardation-interacting protein 1; NUFIP; NUFIP1; NUFIP1, FMR1 interacting protein 1; NUFP1; Rsa1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG