CST, 74076S, PPAR delta (E8O3H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PPAR-delta. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, PPAR delta (E8O3H) Rabbit Monoclonal Antibody (CST #74076) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity PPAR delta (E8O3H) Rabbit Monoclonal Antibody recognizes endogenous levels of total PPARδ protein. This antibody may cross-react with overexpressed PPARα protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human PPARδ protein. Background Peroxisome proliferator-activated receptor-δ (PPARδ, also known as PPARβ or PPARβ/δ) is a widely expressed member of the PPAR nuclear receptor family, which controls lipid homeostasis (1,2). In response to various ligands, PPAR proteins heterodimerize with retinoid X receptors (RXRs) in order to bind DNA and regulate target genes (3,4). PPARδ plays a role in many different biological functions, including cholesterol efflux, embryo implantation, preadipocyte proliferation, and wound healing (5-8). PPARδ has been implicated in colorectal cancer (CRC), as it is normally downregulated by APC, a tumor suppressor frequently knocked out in CRCs (9). More recently, high fat diets have been found to induce PPARδ in intestinal stem cells and progenitors, increasing their tumorigenicity (10). Furthermore, in Huntington's disease (HD) mouse models, it was shown that PPARδ was unable bind to huntingtin protein when mutated, which repressed its function. Agonist-induced activation of PPARδ in HD model mice improved cognitive function and increased survival time (11). Alternate Names FAAR; MGC3931; NR1C2; NUC1; NUCI; NUCII; nuclear hormone receptor 1; nuclear receptor subfamily 1 group C member 2; peroxisome proliferative activated receptor, delta; peroxisome proliferator activated receptor delta; peroxisome proliferator-activated receptor beta; Peroxisome proliferator-activated receptor delta; PPAR-beta; PPAR-delta; PPARB; PPARD; PPARD/MYO1D fusion Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG