CST, 74089S, Cathepsin D (E5V4H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Cathepsin D mouse. Validated for WB,WB,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Cathepsin D (E5V4H) Rabbit Monoclonal Antibody (CST #74089) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunohistochemistry (Paraffin): 1:75 - 1:300 Immunofluorescence (Frozen): 1:800 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:400 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Cathepsin D (E5V4H) Rabbit Monoclonal Antibody detects endogenous levels of preprocathepsin D, procathepsin D, and the heavy chain subunit of mature cathepsin D protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu179 of mouse cathepsin D protein. Background Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (1). It is synthesized as an inactive 43 kDa preprocathepsin D that is cleaved and glycosylated to form a 46 kDa procathepsin D and then further cleaved to produce 28 kDa and 15 kDa subunits (heavy and light chains, respectively) (2). Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway (3). Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularly in breast cancer (4-9). Alternate Names Cat; CatD; Cathepsin D; CD; Ctsd; OTTMUSP00000020357 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 46, 43, 28 Source/Isotype: Rabbit IgG