CST, 74129T, Phospho-Jak1(Tyr1034/1035) (D7N4Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying JAK1 (Tyr1034/Tyr1035) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Jak1(Tyr1034/1035) (D7N4Z) Rabbit Monoclonal Antibody (CST #74129) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Jak1(Tyr1034/1035) (D7N4Z) Rabbit Monoclonal Antibody recognizes endogenous levels of Jak1 protein only when phosphorylated at Tyr1034/1035. This antibody also detects Jak1 when phosphorylated at just Tyr1034. Human Jak1 residues Tyr1034 and Tyr1035 historically have been referenced as Tyr1022 and Tyr1023. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Tyr1034/1035 of human Jak1 protein. Background Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. Alternate Names AIIDE; JAK-1; JAK1; JAK1A; JAK1B; Janus kinase 1; Janus kinase 1 (a protein tyrosine kinase); JTK3; Tyrosine-protein kinase JAK1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG