CST, 74139S, BANK1 (F4G1Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BANK1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, BANK1 (F4G1Q) Rabbit Monoclonal Antibody (CST #74139) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity BANK1 (F4G1Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total BANK1 protein. This antibody may detect bands of unknown origin around 50-60 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala632 of human BANK1 protein. Background B cell scaffold protein with ankyrin repeats (BANK1) is an adaptor protein that is highly expressed in B lineage but not T lineage cells (1). BANK1 is phosphorylated upon B cell antigen receptor (BCR) stimulation and functions as a scaffolding protein, promoting Lyn-mediated phosphorylation of inositol 1,4,5-triphosphate receptor (IP3R) and downstream calcium signaling (1). BANK1 also associates with B lymphoid tyrosine kinase (BLK) and phospholipase C gamma 2 (PLCg2), two additional mediators of B cell signaling (2,3). Knockout studies in mice suggest that BANK1 additionally acts as a negative regulator of CD40-mediated Akt activation (4). gene mutations are associated with several diseases, including systemic lupus erythematosus (SLE), systemic sclerosis, and rheumatoid arthritis (5-7). Alternate Names B cell scaffold protein with ankyrin repeats 1; B-cell scaffold protein with ankyrin repeats; B-cell scaffold protein with ankyrin repeats 1; BANK; BANK1; FLJ20706; FLJ34204 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 105 Source/Isotype: Rabbit IgG