CST, 74180SF, CD15/SSEA1 (MC480) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying FUT4. Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, CD15/SSEA1 (MC480) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #74180) is ready to ship. Product Usage Information This product is the carrier free version of product #4744. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 4744 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD15/SSEA1 (MC480) Mouse Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of SSEA1 antigen. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with F9 teratoma cells (X-irradiated). Background SSEA1 antibody detects a lactoseries oligosaccharide antigen that is expressed on the surface of mouse embryonal carcinoma and embryonic stem cells (1). This antigen is also found on early mouse embryos and both mouse and human germ cells, but is absent on human embryonic stem and carcinoma cells. Expression of SSEA1 in these human cell types increases upon differentiation, while on the mouse cell types differentiation leads to decreased expression (2). Alternate Names alpha (1,3) fucosyltransferase, myeloid-specific; Alpha-(1,3)-fucosyltransferase; Alpha-(1,3)-fucosyltransferase 4; CD15; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; ELFT; FCT3A; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; FucT-IV; FUT4; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA-1; stage-specific embryonic antigen 1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): N/A Source/Isotype: Mouse IgM