CST, 74193S, X-34

Cellular Dyes for studying in the research area. Product Usage Information Supplied Reagents: 1. X-34:Create a 5 mM stock solution of X-34 (200X) using the X-34 diluent. Create a 25 µM staining solution by further diluting the 5 mM stock solution using the X-34 diluent. Note: X-34 diluent (40% EtOH, pH 10) is the recommended solvent for staining. A slight precipitate may be present, but will not interfere with the product's performance. Additional Reagants (Not Supplied) 1. X-34 diluent (40% EtOH, pH 10): Combine 20 mL of EtOH in 29.5 mL of dH 2 O. Add 1 µL of 5M NaOH dropwise until the solution reaches pH 10. Bring the final volume of X-34 diluent to 50 mL with dH 2 O. 2. Differentiation buffer: 50 mM NaOH, 80% EtOH Protocol: 1. Stain slides in 25 µM X-34 staining solution for 30 min at room temperature in the dark. 2. Rinse slides three times in dH 2 O. 3. Incubate for 2 min in the differentiation buffer. 4. Rinse slides three times in dH 2 O. 5. If desired, proceed with immunostaining. 6. Mount and image. Storage Store lyophilized at 4ºC, desiccated. In lyophilized form, the product is stable for 12 months. Background