CST, 7425S, CBP (D9B6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CBP. Validated for WB,IP,ChIP,ChIP,C&T. Available in 2 sizes. Highly specific and rigorously validated in-house, CBP (D9B6) Rabbit Monoclonal Antibody (CST #7425) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 . Western Blotting: 1:1000 Immunoprecipitation: 1:200 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 CUT&Tag: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP, Chromatin IP-seq, CUT&Tag Specificity / Sensitivity CBP (D9B6) Rabbit Monoclonal Antibody recognizes endogenous levels of total CBP protein. This antibody also shows some cross-reactivity with p300 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human CBP protein. Background CBP (CREB-binding protein) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). Phosphorylation of p300 at Ser89 by PKC represses its transcriptional activity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors (3,4). Ser1834 phosphorylation of p300 by Akt disrupts its association with C/EBPβ (5). Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex (6). CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS (7). The role of acetylation of CBP/p300 is of particular interest (2,8). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (9). Alternate Names CBP; CREB binding protein; CREB-binding protein; CREBBP; Histone lysine acetyltransferase CREBBP; KAT3A; MKHK1; Protein-lysine acetyltransferase CREBBP; RSTS; RSTS1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 300 Source/Isotype: Rabbit IgG