CST, 74293S, IgM Antibody

Polyclonal Antibody for studying IGHM. Validated for Western Blotting. Highly specific and rigorously validated in-house, IgM Antibody (CST #74293) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IgM Antibody recognizes endogenous levels of total IgM heavy chain protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of the human IgM heavy chain constant region. Antibodies are purified by protein A and peptide affinity chromatography. Background Immunoglobulin M (IgM) is one of the five major human immunoglobulin isotypes and is the first antibody to be secreted by plasmablasts in a humoral immune response after exposure to antigen. Structurally, IgM is the largest immunoglobulin and predominantly exists in pentameric form when secreted (1). Alternative splicing of the IgM heavy chain mRNA can generate an alternative form of the antibody, which facilitates its insertion into the plasma membrane of B cells to function in antigen recognition. IgM is the first Ig isotype to appear on the surface of developing B cells and is a major component of the B cell antigen receptor (BCR) signaling complex, which drives B cell survival and proliferation upon antigen-induced ligation (2,3). Research studies have shown that defects in the assembly of the BCR account for the low levels of surface IgM expression in B-chronic lymphocytic leukemia (4,5). test This product detects a SARS-CoV-2-related target for research into the mechanisms of the Novel Coronavirus, which has caused the COVID-19 pandemic. Alternate Names AGM1; constant region of heavy chain of IgM; DKFZp686I15196; DKFZp686I15212; FLJ00385; Ig mu chain C region; Ig mu chain C region BOT; Ig mu chain C region GAL; Ig mu chain C region OU; IGHM; IgM; Immunoglobulin heavy constant mu; immunoglobulin mu chain; MGC104996; MGC52291; MU; VH Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 SOURCE: Rabbit