CST, 74315S, SP1 (D4C3) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying SP1. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, SP1 (D4C3) Rabbit Monoclonal Antibody (PE Conjugate) (CST #74315) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity SP1 (D4C3) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total SP1 protein. This antibody conjugate is predicted to detect all three known isoforms. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro593 of human SP1 protein (Isoform 1). Background Specificity protein 1 (SP1) is a ubiquitously expressed transcription factor belonging to the family of C2H2-type zinc finger containing DNA-binding proteins. SP1 binds GC-rich motifs with high affinity and regulates the expression of numerous mammalian genes (1,2). It interacts with many other transcription factors, such as c-Myc, EGR1, and Stat1, and with basal transcription machinery components. SP1 interacts with chromatin-modifying factors, such as histone deacetylases (HDACs) and p300 in chromatin remodeling. Transcriptional activity and stability of SP1 are regulated by post-translational modification, including phosphorylation, acetylation, ubiquitination, and glycosylation (3). Glycosylation of SP1 following insulin treatment leads to increased nuclear localization, while glucagon treatment increases cytoplasmic SP1 levels (4-6). Investigators have found high levels of SP1 in patients with Alzheimer's disease (7). Alternate Names SP1; Sp1 transcription factor; specificity protein 1; Transcription factor Sp1; TSFP1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG