CST, 74355SF, Cathepsin L (E3R3P) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Cathepsin L. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Cathepsin L (E3R3P) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #74355) is ready to ship. Product Usage Information This product is the carrier free version of product #55914. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 55914 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Cathepsin L (E3R3P) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total cathepsin L protein. This antibody detects full-length cathepsin L pro-enzyme in addition to the processed (active) form of cathepsin L. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to a fragment of human cathepsin L protein spanning residues Ala114 to Thr288. Background Cathepsin L belongs to the C1 family of peptidases, a large and diverse family of cysteine (thiol) proteases that play fundamental roles in protein processing and degradation. The catalytic activity of C1 peptidases maps to a nucleophilic cysteine thiol, usually found within a catalytic dyad or triad (1). Cathepsin L, initially synthesized as procathepsin L before undergoing proteolytic cleavage to its active form, is a ubiquitously expressed thiol protease, expressed primarily in lysosomes but which is also secreted by some cell types. Its diverse functions include general protein catabolism and autophagy (2), antigen processing (3), promoting angiogenesis (4), and extracellular matrix degradation (5,6). An isoform of cathepsin L has also been reported in the nucleus, where it was shown to be involved in the regulation of transcription factor activity (7). Targeted deletion of the gene in mice embryos was non-lethal, but resulted in periodic hair loss and skin defects, including epidermal hyperplasia, acanthosis, and hyperkeratosis (8). More recently, research studies have reported that cathepsin L is involved in viral protein processing during viral replication, and that cathepsin L inhibitors may have utility in combating infection by SARS-CoV-2 and related coronaviruses (9). Alternate Names cathepsin L; Cathepsin L1; Cathepsin L1 heavy chain; Cathepsin L1 light chain; CATL; CATL1; CTSL; CTSL1; FLJ31037; Major excreted protein; MEP; Procathepsin L Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 25-42 Source/Isotype: Rabbit IgG