CST, 74467S, Cbl-b (D3C12) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying Cbl-b. Validated for Western Blotting. Highly specific and rigorously validated in-house, Cbl-b (D3C12) Rabbit Monoclonal Antibody (Biotinylated) (CST #74467) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cbl-b (D3C12) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total Cbl-b protein. This antibody does not cross-react with c-Cbl and based upon sequence alignment, is not predicted to cross-react with Cbl-c. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Cbl-b protein. Background The Casitas B lineage lymphoma (Cbl) proteins (in mammals these are c-Cbl, Cbl-b, and Cbl-c) are a family of single subunit RING finger protein-ubiquitin E3 ligases that contain multiple protein interaction motifs (1). All Cbl proteins have a highly conserved N-terminal tyrosine kinase-binding (TKB) domain that mediates interactions between Cbl proteins and phosphorylated tyrosine residues on Cbl substrates. C-terminal to the RING finger, Cbl proteins have proline-rich domains that mediate interactions with SH3 domain-containing proteins. Phosphorylated tyrosine residues mediate interactions with SH2 domain-containing proteins such as the p85 subunit of PI3K. These protein-protein interaction motifs allow Cbl family proteins to function as adaptor proteins (2). This adaptor function contributes to the E3-dependent activities of Cbl proteins by targeting specific substrates for ubiquitination and degradation. The adaptor function also contributes to non-E3-dependent activities, such as the recruitment of proteins involved in receptor tyrosine kinase internalization, localization of Cbl proteins to specific subcellular compartments, and activation of discrete signaling pathways (1). Alternate Names Cas-Br-M (murine) ecotropic retroviral transforming sequence b; Cas-Br-M (murine) ectropic retroviral transforming sequence b; Casitas B-lineage lymphoma proto-oncogene b; Cbl proto-oncogene B; Cbl proto-oncogene B, E3 ubiquitin protein ligase; Cbl proto-oncogene, E3 ubiquitin protein ligase B; Cbl-b; CBLB; DKFZp686J10223; DKFZp779A0729; DKFZp779F1443; E3 ubiquitin-protein ligase CBL-B; FLJ36865; FLJ41152; Nbla00127; RING finger protein 56; RING-type E3 ubiquitin transferase CBL-B; RNF56; SH3-binding protein CBL-B; Signal transduction protein CBL-B Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 125,130 Source/Isotype: Rabbit IgG